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Tag Archives: Zymogram

Protocol for Zymogram

Reagents: 1% Gelatin in H2O (Fisher Blood 275) 1% Casein (Sigma) SDS-PAGE gel stock w/o urea Wash buffer: 2.5% Triton X-100 in H2O (+0.02% NaN3) Incubation Buffer: 50mM Tris-HCl (PH8.0), 5mM CaCl2, 0.02% NaN3 Gels: Regular separating gel containing 10-12% substrate Regular stacking gel Protocol: 1- Collect media from cells (if desired, inactivate non-metalloproteases with…

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Protocol for Zymogram

Reagents: 1% Gelatin in H2O (Fisher Blood 275) 1% Casein (Sigma) SDS-PAGE gel stock w/o urea Wash buffer: 2.5% Triton X-100 in H2O (+0.02% NaN3) Incubation Buffer: 50mM Tris-HCl (PH8.0), 5mM CaCl2, 0.02% NaN3 Gels: Regular separating gel containing 10-12% substrate Regular stacking gel Protocol: 1- Collect media from cells (if desired, inactivate non-metalloproteases with…

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