Tag Archives: western blotting

Western Blot protocol 

Making the gel 1. Use alcohol and Kimwipes to wipe the glass, and then set up the rest of the apparatus 2. For small proteins, use a higher percentage gel 2. Add all ingredients except ammonium persulfate; before adding ammonium persulfate, if the weather is cold in the room, you may wash the solution with…

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Western Blotting Protocol (1)

General Western Blot Protocol General Western Blot Protocol 1. Polyacrylamide gel electrophoresis and blotting a. Add an appropriate amount of electrophoresis sample buffer (1X = 125mM …   Western Blotting Protocol Western Blotting and Immunostaining Protocol. Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents:. Transfer buffer ……

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