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Tag Archives: Western Blot Protocol

Western Blot Protocol

Sample protein preparation Monolayer Cells Grow cells to 80-90% confluency in a 100 mm dish, remove culture medium and rinse cell monolayer with 1x PBS at room temperature. Add 0.3-0.5 ml lysis buffer enriched with protease inhibitor cocktail to the monolayer cells in the plate. Scrape the cells off and transfer the lysate to an…

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