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Tag Archives: nuclear staining

DAPI and PI Stains

DAPI (nucleic acid stain)   100mM stock – 10mg/285.5ml dH2O   Working solution – 300nM (1/333 dilution)   Perform DAPI stain after all other labeling/staining.   Add enough DAPI (1/333) to cover sections.   Incubate for 1-5 minutes.   Rinse several times with 1xPBS.  DISCARD WASTE OVER CHARCOAL.   View with DAPI filter (358/461nm).  …

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