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Tag Archives: mitochondria

Isolation of Mitochondria from Tissue Culture Cells

Abstract: The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1–2 mL derived from ∼109 cells grown in culture. The cells are swollen in…

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