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Tag Archives: Making mutants

Making mutants in Dickeya and Pectobacterium species

Objective: To disrupt a gene in a soft rot bacterial genome. Insertion mutation: an antibiotic resistance gene cassette is inserted into a convinient restriction site in the gene. One major pitfalls with this type of mutant is that the remaining N-terminal portion of the protein is sometimes functional and can even have dominant negative effects.…

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