Tag Archives: fcm

Flow Cytometry: A powerful tool for T cell immunophenotyping

Flow cytometry provides the ability to type immune cells based on their phenotype. The presence of specific cell surface markers, cytokine expression, or phosphorylation of key proteins may be used to immunophenotype specific sub-populations from a heterogeneous starting population. Thus, the unique signature carried by different immune cells can be utilized to isolate cell populations…

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Standard FACS protocol

MIT Synthetic Biology Working Group Standardized FACS Protocol Draft 1 – 9/20/04 This protocol sets out to standardize the components of a FACS experiment necessary to allow repeatable and comparable results. The output of these experiments is typically fluorescence from a genetic regulatory network. Media and Glassware Provisionally, supplemented M9 media will be used for…

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