Tag Archives: Deoxyribose

Degrade DNA to Get Deoxyribose for GC/MS Analysis

Dissolve 100ug DNA in 200ul distilled water. Add MgCL2 and sodium acetate to the final concentration of 15mM and 10mM, respectively. Incubate with 2 units DNAse I at room temperature for 15 minutes. Add ammonium bicarbonate to the final concentration of 100mM, and then incubate with 2mU            phosphodiesterase at 37oC in water bath for 2 hours. Add…

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