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Tag Archives: Deoxyribonucleoside triphosphates

Deoxyribonucleoside triphosphates (dNTPs)

Dissolve each dNTP in distilled water at an approximate concentration of 100 mM. Adjust to pH 7.0 with 0.05 M Tris base using a micropipette (use pH paper to check the pH). Dilute an aliquot of the neutralized dNTP appropriately, and read the optical density at the wavelengths given in the table below. Base                    Wavelength                          Extinction coefficient…

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