Tag Archives: bioprotocol

Standard FACS protocol

MIT Synthetic Biology Working Group Standardized FACS Protocol Draft 1 – 9/20/04 This protocol sets out to standardize the components of a FACS experiment necessary to allow repeatable and comparable results. The output of these experiments is typically fluorescence from a genetic regulatory network. Media and Glassware Provisionally, supplemented M9 media will be used for…

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General Lab Techniques Lab security and basic techniques Advanced lab skills   Molecular Separation & Labeling Molecular Separation Molecular Tracer Labeling   DNA and RNA Nucleic acid methods (1) Microarray protocols.   Genetics Epigenetics protocols Single nucleotide polymorphisms (SNPs)   Molecular Biology–PCR Serials PCR,RT-PCR,Real time PCR etc. PCR (General Procedure)   Molecular Biology–Protein Protein methods Western blot hybridization  …

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Protocol for Zymogram

Reagents: 1% Gelatin in H2O (Fisher Blood 275) 1% Casein (Sigma) SDS-PAGE gel stock w/o urea Wash buffer: 2.5% Triton X-100 in H2O (+0.02% NaN3) Incubation Buffer: 50mM Tris-HCl (PH8.0), 5mM CaCl2, 0.02% NaN3 Gels: Regular separating gel containing 10-12% substrate Regular stacking gel Protocol: 1- Collect media from cells (if desired, inactivate non-metalloproteases with…

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Proper Method for Intraperitoneal Injections

Preferred site: Animal’s lower left abdominal quadrant. Needle size: 21 gauge or smaller (usually use 25 gauge for luciferin injection) Volume: 100ul of luciferin (15mg/ml stock) per 10 grams of mouse body weight. Note: 1ml i.p. injection of a nonirritating solution is easily tolerated. Position of animal: Manually restrained, dorsal recumbency (abdomen side up), with…

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