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Tag Archives: 1 M CaCl2

1 M CaCl2 (200 ml)

Weigh out 54 g CaCl2?H2O and dissolve in 170 ml of distilled water. Make up volume to 200 ml with distilled water. Sterilize the solution by passage through a 0.22-micron filter. <STORAGE> Store in 1 ml aliquots at -20 C. <USAGE> When preparing competent cells, thaw an aliquot and dilute it to 100ml with distilled…

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