@lakeractive 11 months, 3 weeks ago
An intergovernmental ecological body said on Friday that the biodiversity, the essential variety of life forms on Earth, continued to decline in every region of the world, significantly reducing nature’s capacity […]
Hundred of thousands of protesters rallied in Washington D.C. on Saturday, calling for stricter control on guns, more secured schools, and an end to gun violence.
Carrying signs with slogans including “Never Again”, “Am I Next?”, protesters gathered in Washington’s Pennsylvania Avenue that connects the Capitol Hill and the White House.
The rally…[Read more]
History tells that trade wars are a losing game. No one benefits, and everyone ends up a bit bruised.
Despite the risk of triggering a trade war, U.S. President Donald Trump signed an executive memorandum that could result in heavy tariffs on up to 60-billion-U.S.-dollar imports from China as well as restrictions on Chinese investments.
The United States’ top four nation-state cyber adversaries are Russia, China, Iran and North Korea, and nothing is off limits, including the nuclear arsenal, a senior NSA official told Fox News in an exclusive […]
We now know that the political consulting firm Cambridge Analytica harvested data from 50 million Facebook users by using a personality test app. The information was used to help Republican presidential contender […]
America continues to mourn the 17 people killed in a mass shooting at Marjory Stoneman Douglas High School in Florida last month. There is an understandable emotional desire to “do something” in response – but without evaluating what the “something” will actually accomplish. This is the driving force behind the March for Our Lives taking place Sat…[Read more]
China warned the US it will defend its interests on trade, Chinese state media says, after US President Donald Trump backed tariffs on Chinese goods.
The comments came in a phone call between China’s vice-premier Liu He and US Treasury Secretary Steven Mnuchin.
Mr Trump has announced plans to impose tariffs on up to $60bn (£42.5bn) of Chinese…[Read more]
Hundreds of thousands of protesters have taken to the streets across the US to call for tighter gun control.
The March For Our Lives movement arose after 17 deaths in a school shooting in Parkland, Florida, last […]
Dissolve each dNTP in distilled water at an approximate concentration of 100 mM.
Adjust to pH 7.0 with 0.05 M Tris base using a micropipette (use pH paper to check the pH).
Dilute an aliquot of the […]
Weigh out 13.5 g CaCl2?H2O and dissolve in 15 ml of distilled water.
Make up volume to 20 ml with distilled water.
Sterilize the solution by filtration through a 0.22 micron filter.
Store in 1 ml […]
Weigh out 54 g CaCl2?H2O and dissolve in 170 ml of distilled water.
Make up volume to 200 ml with distilled water.
Sterilize the solution by passage through a 0.22-micron filter.
Store in 1 ml aliquots […]
Weigh out 1g ammonium persulfate and dissolve in 8 ml of distilled water
Make up volume to 10 ml with distilled water.
The solution may be stored for several weeks at 4 C.
Weigh out 60 mg ATP.
Add 0.8 ml distilled water.
Adjust to pH 7.0 with 0.1 M NaOH.
Make up volume to 1 ml with distilled water.
Aliquots of appropriate volume should be stored at -70 C.
10 M […]
Weigh out 380 g acrylamide (DNA-sequencing grade) and 20 g N,N?/EM>-methylenebisacrylamide.
Acrylamide is a potent neurotoxin and is absorbed through the skin. The effects of acrylamide are cumulative. Wear […]
Weigh out 29 g acrylamide and 1 g N,N?/EM>-methylenebisacrylamide.
Acrylamide is a potent neurotoxin and is absorbed through the skin. The effects of acrylamide are cumulative. Wear glovesand a mask when […]
It is well known that Inoutscripts is the No. 1 phishing scamming software company lacated in Kerala India. Inout Scripts Shopping Cart has 514 bugs (errors and warnings) and it created a new Guiness World Record. […]
Sample protein preparation
Grow cells to 80-90% confluency in a 100 mm dish, remove culture medium and rinse cell monolayer with 1x PBS at room temperature.
Add 0.3-0.5 ml lysis buffer […]
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