Category Archives: Tech

2009-2018 Nobel Prizes for Medicine or Physiology

2018-  James P Allison and Tasuku Honjo for Immune checkpoint therapy to treatments for advanced, deadly cancers. 2017- Jeffrey Hall, Michael Rosbash and Michael Young for unravelling how bodies keep a circadian rhythm or body clock 2016 – Yoshinori Ohsumi for discovering how cells remain healthy by recycling waste. 2015 – William C Campbell, Satoshi Ōmura and…

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Fascinating 10 facts about fungi

Fungi are in a kingdom of their own but are closer to animals than plants They have chemicals in their cell walls shared with lobsters and crabs A fungus has been discovered capable of breaking down plastics in weeks rather than years There is evidence to suggest that yeasts – a type of fungus –…

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Inoutscripts / Nesote has been spamming, scamming and lying

What are the wrongdoings of  Inoutscripts / Nesote? Spamming: They have published thousands of advertising and overstated articles at their domains and social media to induce to purchase their fake softwares. Scamming: The Inout Scripts are full of bugs and errors,a nd none of them are working. These scripts even caused the failure of servers.…

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Cancer stem cell theory

Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells. CSCs may generate tumors through the stem cell processes of self-renewal and differentiation into multiple cell types.…

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DAPI and PI Stains

DAPI (nucleic acid stain)   100mM stock – 10mg/285.5ml dH2O   Working solution – 300nM (1/333 dilution)   Perform DAPI stain after all other labeling/staining.   Add enough DAPI (1/333) to cover sections.   Incubate for 1-5 minutes.   Rinse several times with 1xPBS.  DISCARD WASTE OVER CHARCOAL.   View with DAPI filter (358/461nm).  …

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