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Category Archives: Tech

Fascinating 10 facts about fungi

Fungi are in a kingdom of their own but are closer to animals than plants They have chemicals in their cell walls shared with lobsters and crabs A fungus has been discovered capable of breaking down plastics in weeks rather than years There is evidence to suggest that yeasts – a type of fungus –…

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Inoutscripts / Nesote has been spamming, scamming and lying

What are the wrongdoings of  Inoutscripts / Nesote? Spamming: They have published thousands of advertising and overstated articles at their domains and social media to induce to purchase their fake softwares. Scamming: The Inout Scripts are full of bugs and errors,a nd none of them are working. These scripts even caused the failure of servers.…

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Cancer stem cell theory

Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells. CSCs may generate tumors through the stem cell processes of self-renewal and differentiation into multiple cell types.…

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DAPI and PI Stains

DAPI (nucleic acid stain)   100mM stock – 10mg/285.5ml dH2O   Working solution – 300nM (1/333 dilution)   Perform DAPI stain after all other labeling/staining.   Add enough DAPI (1/333) to cover sections.   Incubate for 1-5 minutes.   Rinse several times with 1xPBS.  DISCARD WASTE OVER CHARCOAL.   View with DAPI filter (358/461nm).  …

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PCR Based Moleclar Cloning

In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add overhangs to your…

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