DAPI (nucleic acid stain)
- 100mM stock – 10mg/285.5ml dH2O
- Working solution – 300nM (1/333 dilution)
- Perform DAPI stain after all other labeling/staining.
Add enough DAPI (1/333) to cover sections.
Incubate for 1-5 minutes.
Rinse several times with 1xPBS. DISCARD WASTE OVER CHARCOAL.
- View with DAPI filter (358/461nm).
Propidium Iodide (PI, nucleic acid stain)
- Incubate sample for 20 min at 37C in 2xSSC+100mg/ml RNase.
- Rinse sections 3x for 1 min each in 2xSSC.
- Equilibrate sections in 2xSSC.
- Dilute PI 1/3000 in 2xSSC. Incubate for 1-5 min.
- Rinse several times with 2x SSC.