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LAL Endotoxin Assay

Preparing Standards

  1. Prepare 1 EU/ml standard
  • Place 0.1 ml of endotoxin stock solution into a 1.7 ml microfuge tube.
  • Add LAL Reagent water.

Calculate water addition as follows:

ml water = (X-1) / 10

x is equal to the concentration of endotoxin in the stock solution.

  • Vortex vigorously for 1 min
  1. 0.5 EU/ ml standard
  • Combine 0.5 ml 1 EU/ml standard and 0.5 ml LAL Reagent water into a 1.7 ml microfuge tube.
  • Vortex vigorously for 1 min.
  1. 0.25 EU/ ml standard
  • Combine 0.25 ml 1 EU/ml standard and 0.75 ml LAL Reagent water into a 1.7 ml microfuge

tube.

  • Vortex vigorously for 1 min.
  1. 0.1 EU/ ml standard
  • Combine 0.1 ml 1 EU/ml standard and 0.9 ml LAL Reagent water into a 1.7 ml microfuge tube.
  • Vortex vigorously for 1 min.

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