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Common Fixatives Used for Immunohistochemistry

(1) 100% acetone. Pre-cold in a fridge. Cryosections are prepared, dry at room temperature overnight. Fixed with cold acetone for 20 min.

(2) 4% paraformaldehyde in 0.1M phosphate buffer. Tissue can be cut into 3 × 3 × 6 mm in size; fixed for 12 h at room temperature. Dry cryosections can be fixed at room temperature for 1 h.

(3) 2% paraformaldehyde with 0.2% picric acid in 0.1M phosphate buffer. Fixation is the same as (2).

(4) PLP fixative: 4% paraformaldehyde, 0.2% periodate and 1.2% lysine in 0.1M phosphate buffer. Fixation is the same as (2).

(5) 4% paraformaldehyde with 0.05% glutaraldehyde (TEM immunohistochemistry). Tissue is cut into 2 × 2 × 4 mm, fixed for 2 h at 4°C.

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