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MicroRNA and siRNA Cloning Method

1. Oligo Design
2. ImpA Synthesis
3. Synthesizing Oligos
4. Oligonucleotide Adenylation
5. Small RNA Purification
6. 3′ Adaptor Ligation
7. 5′ Adaptor Ligation
8. RT-PCR
9. Concatamerization
10. Cloning into Topo vector
11. Screen and Sequence

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