Standard FACS protocol
MIT Synthetic Biology Working Group Standardized FACS Protocol Draft 1 – 9/20/04
This protocol sets out to standardize the components of a FACS experiment necessary to allow repeatable and comparable results. The output of these experiments is typically fluorescence from a genetic regulatory network.
Media and Glassware
Provisionally, supplemented M9 media will be used for all cultures. The media recipe used by CC and JCB is –
- 1x M9 Minimal Salts (from Sigma):
- 12.8 g/L NaHPO4.7H20
- 3.0 g/L KH2PO4
- 0.5 g/L NaCl
- 1.0 g/L NH4Cl
- 0.1mM MgSO4
- 2mM CaCl2
- 0.4% Glucose/Glycerol
- 0.1% Casamino Acids
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