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Phosphorylation Assay

Materials required:

Cells

PBS

HAM F-12 media

Growth factor (VEGF)

Lysis Buffer mix (w/o PMSF and Aprotinin, keep at 4C, add PMSF and

Aprotinin right before use):

1% Triton-X100

10mM Tris

1mM EDTA

150mM NaCl

30mM PPiNa

50mM NaFi

2.1mM Na3VO4

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